CHEM 433LAB - Biochemistry Lab II

Description:
Molecular cloning of a gene into a plasmid vector, followed by expression and purification of the encoded protein using modern chromatography techniques. Downstream analysis and experimentation is then conducted on the purified protein. Six hours of laboratory weekly. Course will be offered every year (Spring).

Prerequisites:
Prerequisite: CHEM 431LAB. Co-or prerequisite: CHEM 433.

Credits: (2)

Learner Outcomes:
Upon successful completion of this course, the student will be able to:

Clone a gene either by the polymerase chain reaction or by cut and paste with restriction endonucleases.
Express and purify a protein by column chromatography.
Design experiments to characterize a protein.
Calculate data and organize results in tabular or graphical format.

Learner Outcomes Approval Date:
1/20/11

Anticipated Course Offering Terms and Locations: